By perfusing living heart tissue with voltage or calcium sensitive dyes, it is possible to use light to measure the electrical activity corresponding to functional activity. While 2D optical imaging of the outer surface of the heart using voltage sensitive dyes is fairly well established, it is a significant challenge to be able to image the propagation of electrical waves that are not traveling in a plane perpendicular to the heart wall.
In this project, we are working with collaborators at SUNY upstate medical university to develop optical imaging methods capable of imaging the transmural propagation of light in the living, perfused heart. So far, we have applied Laminar Optical Tomography measurement techniques to image the voltage sensitive fluorescence changes corresponding to endocardial and epicardial stimulation.
Hillman E. M. C, Bernus O, Pease E, Bouchard M. B. Pertsov A. “Depth-resolved optical imaging of transmural electrical propagation in perfused heart”, Optics Express, 15 (26), 17827-17841, 2007.
Bouchard M. B, Burgess S. A, Moussazadeh P, Radosevich AJ, Wuskell JP, Loew LM, Pertsov A, Hillman E. M. C. Electrical and metabolic imaging of cardiac ischemia In: OSA Biomedical Topical Meetings, OSA Technical Digest, Optical Society of America, Washington DC, 2008. March 16-19; St Petersberg FL.
Burgess S. A, Yuan B, Pease E, Iranmahboob A, Hillman E. M. C. Laminar Optical Tomography for Dermal and Cardiac Imaging. Engineering Conferences International: Advances in Optics for Biotechnology, Medicine and Surgery, Naples, June 2007.
Hillman E. M. C, Devor A, Dunn AK, Boas DA “Laminar Optical Tomography: High-resolution 3D functional imaging of superficial tissues“ [Invited] In: Medical Imaging, SPIE, San Diego, CA (2006)
Hillman E. M. C, Devor A, Boas DA “High-resolution functional optical imaging of living tissues“ [Invited] In: IEEE International Symposium of Biomedical Imaging Medical Imaging (ISBI) Arlington, VA (2006)
Hillman, E. M. C, Boas, D. A, Dale, A. M, Dunn, A. K, "Laminar Optical Tomography: demonstration of millimeter-scale depth-resolved imaging in turbid media", Optics Letters, 29, (14), 1650-1652 (2004).